KAPA Library Preparation

The KAPA Library Preparation Kit provides all of the enzymes and reaction buffers required for constructing Illumina libraries from fragmented dsDNA. Reaction buffers are supplied with all of the required reaction components except oligonucleotide adaptors or PCR primers. Similarly, a single enzyme mixture is provided for each step of the library construction process, reducing the number of pipetting steps.

The KAPA Library Preparation Kit contains the following modules:

End Repair: The End Repair module produces blunt-ended, 5'-phosphorylated DNA fragments and contains an enzyme mix (T4 DNA Polymerase and T4 Polynucleotide Kinase) and buffer with dNTPs.

A-Tailing: The A-Tailing module adds dAMP to the 3'-ends of the dsDNA fragments and contains Klenow fragment and Buffer.

Adaptor Ligation: The Ligation module ligates dsDNA adaptors with 3'-dTMP overhangs to library fragments and contains DNA ligase and ligation buffer. 

Library Amplification: The amplification module uses high fidelity PCR to selectively amplify library fragments carrying appropriate adaptor sequences prior to sequencing. The KAPA Library Preparation Kit is supplied with either standard end-point or real-time PCR amplification module. End-Point PCR module contains KAPAHiFi Library Amp ReadyMix. The real-time PCR module contains KAPAHiFi Library Amp Real-Time with SYBR Green I dye and four fluorescent reference standards representing a range of distinct DNA concentrations.

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