PEQLAB Biotechnologie GmbH • Carl-Thiersch-Str. 2b • D-91052 Erlangen
Tel. +49(0)9131 / 610 7020 • Fax +49(0)9131 / 610 7099
E-Mail: info@peqlab.de • Web: http://www.peqlab.de
Tel. +49(0)9131 / 610 7020 • Fax +49(0)9131 / 610 7099
E-Mail: info@peqlab.de • Web: http://www.peqlab.de
Uracil DNA Glycosylase
After incorporation of uracil into the DNA strand, UDG catalyses the hydrolysis of the n-glycosidic bond between uracil and deoxyribose. Before starting the PCR an incubation at 37 °C for 10 minutes degrades uracil containing DNA fragments from previous experiments. The enzyme is deactivated by heat shock at 95 °C for 10 minutes.
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